研究组有工作人员1名，研究生人数维持在5-8名。

采用生物化学、结构生物学方法，研究与癌症、神经退行性疾病相关蛋白质的结构与功能；以这些蛋白质为靶标，设计全新化学结构的抑制剂或激动剂，为药物研发提供新的先导化合物；发展蛋白质结构功能研究、药物分子设计的新方法和新技术。目前的工作涉及Bcl-2家族蛋白、Ab和蛋白去乙酰化酶。

1. Luo L, Yang J, Liu D. Integrationand oligomerization of Bax protein in lipid bilayers characterized by single molecule fluorescence study. Journal of Biological Chemistry. 2014, 289(46):31708-18. 

2. Wu J, Li Y, Chen K, Jiang H, Xu MH, Liu D. Identification of benzofuran-3-yl (phenyl)methanones as novel SIRT1 inhibitors: binding mode, inhibitory mechanism and biological action. European Journal of Medicinal Chemistry. 2013, 60:441-50. 

3. Wu J, Zhang D, Chen L, Wang J, Ning C, Chen D, Yu N, Chen K, Jiang H, Liu H, Liu D. Discovery and Mechanism Study of SIRT1 Activators that Promote the Deacetylation of Fluorophore-Labeled Substrate. Journal of Medicinal Chemistry. 2013, 56(3):761-80. 

4. Zhou Y, Jiang C, Zhang Y, Liang Z, Liu W, Wang L, Luo C, Zhong T, Sun Y, Zhao L, Xie X, Jiang H, Zhou N, Liu D*, Liu H*. Structural Optimization and Biological Evaluation of Substituted Bisphenol A Derivatives as b-Amyloid Peptide Aggregation Inhibitors. Journal of Medicinal Chemistry. 2010, 53(15):5449-66.

5. Feng Y, Ding X, ChenT, Chen L, Liu F, Jia X, Luo X, Shen X, Chen K, Jiang H, Wang H*, LiuH*, Liu D*. Design, Synthesis and Interaction Study of Quinazoline-2(1H)-Thione Derivatives as Novel Potential Bcl-xL Inhibitors. Journal of Medicinal Chemistry, 2010, 53:3465-79.
6. Jiang C, Feng Y, Huang X, Xu Y, Zhang Y, Zhou N, Shen X, Chen K, Jiang H*, Liu D*. An enzyme-linked immunosorbent assay to compare the affinity of chemical compounds for b-amyloid peptide as a monomer. Analytical and Bioanalytical Chemistry. 2010, 396:1745-54.
7. Chen L, Feng Y, Zhou Y, Zhu W, Shen X, Chen K, Jiang H, Liu D*. Dual role of Zn2+ in maintaining structural integrity and suppressing deacetylase activity of SIRT1. Journal of Inorganic Biochemistry. 2010, 104(2):180-5.
8. Feng Y, Liu D*, Shen X, Chen K, Jiang H. Structure assembly of Bcl-xL through a5-a5 and a6-a6 interhelix interactions in lipid membranes. Biochimica et Biophysica Acta-Biomembranes. 2009, 1788(11):2389-95.
9. Feng Y, Wu J, Chen L, Luo C, Shen X, Chen K, Jiang H, Liu D*. A fluorometric assay of SIRT1 deacetylation activity through quantification of nicotinamide adenine dinucleotide. Analytical Biochemistry. 2009, 395(2):205-10.
10. Feng Y, Zhang L, Hu T, Shen X, Ding J, Chen K, Jiang H, Liu D*. A conserved hydrophobic core at Bcl-xL mediates its structural stability and binding affinity with BH3-domain peptide of pro-apoptotic protein. Archives of Biochemistry and Biophysics. 2009, 484(1):46-54.
11. Feng Y, Shen X, Chen K, Jiang H, Liu D*. A new assay based on fluorescence resonance energy transfer to determine the binding affinity of Bcl-xL inhibitors. Bioscience Biotechnology and Biochemistry. 2008, 72(7):1936-9.
12. Feng Y, Lin Z, Shen X, Chen K, Jiang H, Liu D*. Bcl-xL forms two distinct homodimers at non-ionic detergents: implications in the dimerization of Bcl-2 family proteins. Journal of Biochemistry. 2008, 143(2):243-52.
13. Li Y*, Liu D*, Cao R, Kumar S, Dong C, An J, Wilson SR, Gao YG, Huang Z. Crystal structure of chemically synthesized vMIP-II. Proteins: Structure, Function, and Bioinformatics. 2007, 67(1):243-6.
14. Liu D, Madani N, Li Y, Cao R, Choi WT, Kawatkar SP, Lim MY, Kumar S, Dong CZ, Wang J, Russell JD, Lefebure CR, An J, Wilson S, Gao YG, Pallansch LA, Sodroski JG, Huang Z. Crystal structure and structural mechanism of a novel anti-human immunodeficiency virus and D-amino acid-containing chemokine. Journal of Virology. 2007, 81(20):11489-98.
15. Liu D*, Xu Y, Feng Y, Liu H, Shen X, Chen K, Ma J, Jiang H*. Inhibitor discovery targeting the intermediate structure of b-amyloid peptide on the conformational transition pathway: implications in the aggregation mechanism of b-amyloid peptide. Biochemistry. 2006, 45(36):10963-72.
16. Mori M*, Liu D*, Kumar S, Huang Z. NMR structures of anti-HIV D-peptides derived from the N-terminus of viral chemokine vMIP-II. Biochemical And Biophysical Research Communications, 2005, 335(3):651-8. (SCI, IF 2.648) 
17. Liu D*, Yang B*, Cao R, Huang Z. A chemical strategy to promote helical peptide-protein interactions involved in apoptosis. Bioorganic & Medicinal Chemistry Letters, 2005, 15(20):4467-9.
18. Yang B*, Liu D*, Huang Z. Identification of Affinity-Enhancing Motifs on Pro-apoptosis peptides derived from the BH3 domain of Bcl-2 family proteins. Bioorganic & Medicinal Chemistry Letters, 2004, 14(6):1403-6.
19. Liu D*, Huang Z. Synthetic peptides and non-peptidic molecules as probes of structure and function of Bcl-2 family proteins and modulators of apoptosis. Apoptosis, 2001, 6: 453-62.
20. Wang JL*, Liu D*, Zhang ZJ, Shan S, Han X, Srinivasula SM, Croce CM, Alnemri ES, Huang Z. Structure-based discovery of an organic compound that binds Bcl-2 protein and induces apoptosis of tumor cells. Proceedings of The National Academy of Sciences of The United States of America. 2000, 97:7124-9.

配备生物化学、结构生物学实验的常用仪器设备，主要仪器有荧光光度计、酶标仪、FPLC蛋白质纯化仪、MX5精密分析天平、生物分光光度计、单分子荧光成像系统等。

FPLC蛋白质纯化仪		酶标仪
单分子荧光成像系统		荧光光度计

 

研究室组织结构	刘东祥课题组/丁 侃课题组/王贺瑶课题组/杨财广课题组
	蓝乐夫课题组